The C terminus of MINT forms homodimers and abrogates MINT-mediated transcriptional repression

Junlin Li; Junfeng Li; Xi Yang; Hongyan Qin; Peng Zhou; Yingmin Liang; Hua Han

doi:10.1016/j.bbaexp.2005.02.001

The C terminus of MINT forms homodimers and abrogates MINT-mediated transcriptional repression

Biochim Biophys Acta. 2005 May 25;1729(1):50-6. doi: 10.1016/j.bbaexp.2005.02.001. Epub 2005 Feb 17.

Authors

Junlin Li¹, Junfeng Li, Xi Yang, Hongyan Qin, Peng Zhou, Yingmin Liang, Hua Han

Affiliation

¹ Department of Medical Genetics and Developmental Biology, State Key Laboratory of GI Cancer Biology, Fourth Military Medical University, Xian 710032, China.

PMID: 15777657
DOI: 10.1016/j.bbaexp.2005.02.001

Abstract

Notch signaling plays a pivotal role in numerous cell fate determination events during development, and therefore its regulation has been studied intensively. MSX2-interacting nuclear target protein (MINT) modifies the Notch signaling by interacting with and inhibiting the downstream transcription factor RBP-J/CBF-1 of Notch. In this study, by a yeast two hybrid screening, we found that the C terminal fragment of MINT interacted with each other. We confirmed the interaction between two MINT C terminal fragments both in vitro and in vivo. We further demonstrated that the overexpression of the C terminal fragment of MINT cancelled its inhibitory effect on the transactivation of an RBP-J-dependent promoter by Notch. These results suggest that MINT may form a dimer or multimer in cells through its C terminus, and that the C terminal fragment of MINT may work as its dominant-negative version.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing
Animals
COS Cells
Chlorocebus aethiops
DNA-Binding Proteins / biosynthesis*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Gene Expression Regulation*
Homeodomain Proteins
Humans
Immunoglobulin J Recombination Signal Sequence-Binding Protein
Membrane Proteins / metabolism
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Nuclear Proteins / biosynthesis*
Nuclear Proteins / genetics
Promoter Regions, Genetic
Protein Structure, Tertiary / genetics
Protein Structure, Tertiary / physiology
RNA-Binding Proteins
Receptors, Notch
Signal Transduction / genetics
Signal Transduction / physiology*
Transcription, Genetic / physiology*

Substances

APBA1 protein, human
Adaptor Proteins, Signal Transducing
DNA-Binding Proteins
Homeodomain Proteins
Immunoglobulin J Recombination Signal Sequence-Binding Protein
MSX2 protein
Membrane Proteins
Nerve Tissue Proteins
Nuclear Proteins
RBPJ protein, human
RNA-Binding Proteins
Receptors, Notch
Spen protein, mouse