Optimized DNA targeting using N,N-bis(2-pyridylmethyl)-beta-alanyl 2'-amino-LNA

Chem Commun (Camb). 2005 Apr 7:(13):1705-7. doi: 10.1039/b417101b. Epub 2005 Feb 3.

Abstract

Incorporation of N,N-bis(2-pyridylmethyl)-beta-alanyl 2'-amino-LNA (bipyridyl-functionalized 2'-amino locked nucleic acid) monomers into DNA strands enables high-affinity targeting of complementary DNA with excellent Watson-Crick selectivity in the presence of divalent metal ions. Positioning of bipyridyl-functionalized 2'-amino-LNA monomers in two complementary DNA strands in a "3'-end zipper" constitution allows modulation of duplex stability, i.e., a strong stabilizing effect with one equivalent of divalent metal ion per bipyridyl pair, or a strong destabilizing effect with an excess of divalent metal ions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / chemistry*
  • Amination
  • Cations, Divalent / chemistry
  • DNA / chemistry*
  • Metals, Heavy / chemistry
  • Methylation
  • Models, Molecular
  • Molecular Structure
  • Nucleic Acid Denaturation
  • Pyridines / chemistry*
  • Transition Temperature

Substances

  • Cations, Divalent
  • Metals, Heavy
  • Pyridines
  • DNA
  • pyridine
  • Alanine