Abstract
It is well documented that N-acetyltransferase (NAT) plays a key role in the N-acetylation of arylamine compounds. Ellagic acid was demonstrated to elicit dose-dependent bacteriostatic activity and inhibition of N-acetylation of 2-aminofluorene (AF). N-acetylation of AF in S. aureus was determined by high preformance liquid chromatography. The apparent values of Km and Vmax of NAT were decreased after co-treatment with 0.5 mM ellagic acid in the cytosol of S. aureus. PCR also indicated that ellagic acid inhibited NAT gene expression (NAT mRNA) in S. aureus.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation / drug effects
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Arylamine N-Acetyltransferase / drug effects*
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Arylamine N-Acetyltransferase / genetics
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Arylamine N-Acetyltransferase / metabolism
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Chromatography, High Pressure Liquid
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Dose-Response Relationship, Drug
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Ellagic Acid / pharmacology*
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Enzyme Inhibitors / pharmacology*
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Fluorenes / analysis
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Fluorenes / antagonists & inhibitors
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Gene Expression Regulation, Enzymologic / drug effects*
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Growth Inhibitors / pharmacology*
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Kinetics
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Polymerase Chain Reaction
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / enzymology
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Staphylococcus aureus / growth & development
Substances
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Enzyme Inhibitors
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Fluorenes
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Growth Inhibitors
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Ellagic Acid
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2-aminofluorene
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Arylamine N-Acetyltransferase