Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 May 5;819(1):59-66. doi: 10.1016/j.jchromb.2005.01.022.

Abstract

The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-alpha-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Alpha-Globulins / analysis
  • Amino Acid Sequence
  • Breast Neoplasms / blood*
  • Chromatography, High Pressure Liquid / methods*
  • Female
  • Fibrinogen / analysis
  • Gene Expression Profiling
  • Humans
  • Lasers
  • Male
  • Mass Spectrometry / methods*
  • Melanoma / blood*
  • Neoplasm Proteins / blood*
  • Peptides / blood*
  • Proteomics / methods*
  • Skin Neoplasms / blood*

Substances

  • Alpha-Globulins
  • Neoplasm Proteins
  • Peptides
  • inter-alpha-inhibitor
  • Fibrinogen