Functional, cellular and molecular aspects of skeletal muscle recovery after injury induced by snake venom from Notechis scutatus scutatus

Toxicon. 2005 May;45(6):789-801. doi: 10.1016/j.toxicon.2005.02.001.

Abstract

We have analysed the rate and ultimate extent of muscle functional recovery after snake venom-induced myotoxicity, as well as the relationships between functional, biochemical and structural indices of recovery. We also compared the effects of various injuries leading to muscle necrosis, loss of innervation/vasculature and/or precursors of muscle cells (pmc). We found that several parameters of rat soleus muscle such as maximal isometric force, slow myosin heavy chain, and citrate synthase, were fully and rapidly restored within 6 weeks after treatment with snake Notechis scutatus venom (im, 2 microg/muscle). In contrast, some muscle contractile properties (degree of tetanic fusion, fatigue resistance...) were not fully recovered even by 12 weeks after venom treatment. However, when compared to other injuries, recovery 3 weeks after venom treatment, was better than that observed after severing the terminal nerve and accompanying vessels and after cryodamage known to kill pmc. In conclusion, our studies demonstrate that-contrary to what is commonly believed -- muscle treated by myotoxic agent does not recover rapidly and fully. However, the degree or rate of muscle recovery after snake venom treatment was much better when compared to other types of injury. In addition, histological and biochemical parameters cannot be used as such to easily predict functional recovery following injury.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Citrate (si)-Synthase / metabolism
  • DNA Primers
  • Elapid Venoms / toxicity*
  • Elapidae*
  • Electrophoresis, Polyacrylamide Gel
  • Histological Techniques
  • Immunoblotting
  • Male
  • Muscle Contraction / drug effects
  • Muscle, Skeletal / drug effects*
  • Muscle, Skeletal / pathology
  • Myosin Heavy Chains / genetics
  • Myosin Heavy Chains / metabolism
  • Necrosis / chemically induced
  • Necrosis / pathology
  • Phosphopyruvate Hydratase / metabolism
  • Rats
  • Rats, Wistar
  • Recovery of Function / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Snake Bites / pathology*
  • Time Factors

Substances

  • DNA Primers
  • Elapid Venoms
  • Citrate (si)-Synthase
  • Myosin Heavy Chains
  • Phosphopyruvate Hydratase