Direct detection of Nocardia spp. in clinical samples by a rapid molecular method

Andrée Couble; Verónica Rodríguez-Nava; Michèle Pérouse de Montclos; Patrick Boiron; Frédéric Laurent

doi:10.1128/JCM.43.4.1921-1924.2005

Direct detection of Nocardia spp. in clinical samples by a rapid molecular method

J Clin Microbiol. 2005 Apr;43(4):1921-4. doi: 10.1128/JCM.43.4.1921-1924.2005.

Authors

Andrée Couble¹, Verónica Rodríguez-Nava, Michèle Pérouse de Montclos, Patrick Boiron, Frédéric Laurent

Affiliation

¹ UMR CNRS 5557, Center for Microbial Ecology, Opportunistic Pathogens and Environment Research Group, Observatoire Français des Nocardioses (OFN), Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France.

Abstract

We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.

Publication types

Evaluation Study

MeSH terms

Biopsy
Bronchoalveolar Lavage Fluid / microbiology
Culture Media
DNA, Bacterial / analysis*
DNA, Ribosomal / analysis
Humans
Nocardia / genetics
Nocardia / isolation & purification*
Nocardia Infections / diagnosis*
Nocardia Infections / microbiology
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics*
Sensitivity and Specificity
Skin / microbiology
Sputum / microbiology
Suppuration / microbiology
Time Factors

Substances

Culture Media
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S