Abstract
A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anthrax / diagnosis*
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Anthrax / microbiology
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Bacillus anthracis / classification*
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Bacillus anthracis / genetics
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Bacillus anthracis / isolation & purification
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Bacterial Proteins / genetics*
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Bacterial Typing Techniques*
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Base Sequence
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Codon, Nonsense
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DNA, Bacterial / analysis
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Humans
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Molecular Sequence Data
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Polymorphism, Single Nucleotide / genetics*
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Sequence Analysis, DNA
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Taq Polymerase / metabolism
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Trans-Activators / genetics*
Substances
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Bacterial Proteins
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Codon, Nonsense
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DNA, Bacterial
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PlcR protein, Bacillus
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Trans-Activators
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Taq Polymerase