Abstract
The accuracy and performance of four automated instruments (BD Phoenix, MicroScan WalkAway, VITEK-2 and Wider) were evaluated for susceptibility testing of fluoroquinolones and beta-lactams with four clinical isolates of Klebsiella pneumoniae and the corresponding Escherichia coli transconjugants containing a plasmid carrying the qnr gene and coding for FOX-5 production. No major or very major errors were detected with the MicroScan system. Many of the minor errors for both quinolones and beta-lactams clustered around the intermediate breakpoints.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Automation / standards
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Bacterial Proteins / genetics
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Drug Resistance, Bacterial / genetics
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Escherichia coli / drug effects
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Fluoroquinolones / pharmacology*
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Humans
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / drug effects*
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Microbial Sensitivity Tests / standards*
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Reagent Kits, Diagnostic / standards
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Transfection
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beta-Lactamases / genetics*
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beta-Lactams / pharmacology*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Escherichia coli Proteins
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Fluoroquinolones
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Qnr protein, E coli
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Qnr protein, Klebsiella pneumoniae
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Reagent Kits, Diagnostic
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beta-Lactams
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beta-lactamase FOX-5
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beta-Lactamases