A novel RNA pentaloop fold involved in targeting ADAR2

RNA. 2005 May;11(5):592-7. doi: 10.1261/rna.7276805.

Abstract

Adenosine deaminases that act on RNA (ADARs) catalyze the site-specific conversion of adenosine to inosine in primary mRNA transcripts, thereby affecting coding potential of mature mRNAs. Structural determinants that define the adenosine moieties for specific ADARs-mediated deaminations are currently unknown. We report the solution structure of the central region of the human R/G stem-loop pre-mRNA, a natural ADAR2 substrate encoding the subunit B of the glutamate receptor (R/G site). The structure reveals that the GCU(A/C)A pentaloop that is conserved in mammals and birds adopts a novel fold. The fold is stabilized by a complex interplay of hydrogen bonds and stacking interactions. We propose that this new pentaloop structure is an important determinant of the R/G site recognition by ADAR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / metabolism*
  • Base Sequence
  • Conserved Sequence / genetics
  • Humans
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Nucleic Acid Conformation*
  • Protein Subunits / genetics
  • RNA Precursors / chemistry*
  • RNA Precursors / genetics
  • RNA Precursors / metabolism*
  • RNA-Binding Proteins
  • Receptors, Glutamate / chemistry
  • Receptors, Glutamate / genetics
  • Substrate Specificity

Substances

  • Protein Subunits
  • RNA Precursors
  • RNA-Binding Proteins
  • Receptors, Glutamate
  • ADARB1 protein, human
  • Adenosine Deaminase

Associated data

  • PDB/1YSV