Abstract
A recombinant plasmid pBMBZGC10 was obtained by the ligation of gfp-cry1Ac10 fusion gene and vector plasmid pAD4412, which was then introduced by gene pulser into acrystalliferous strain CryB, and a recombinant strain CryB(pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for multi-spraying on Brassica pekinesis, Ipomoea aquatica and Lycopersicon esculentum leaves. The results of fluorescent detection and PCR amplification revealed that cry1Ac10 gene did not transfer into indigenous bacteria, actinomyces and fungi in test soil, and could not be detected in roots, stems and leaves of test plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus thuringiensis / genetics*
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Bacillus thuringiensis / metabolism
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Bacillus thuringiensis Toxins
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Bacterial Toxins / biosynthesis
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Bacterial Toxins / genetics*
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Endotoxins / biosynthesis
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Endotoxins / genetics*
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Gene Transfer, Horizontal
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Green Fluorescent Proteins / genetics
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Hemolysin Proteins / biosynthesis
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Hemolysin Proteins / genetics*
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Pest Control, Biological*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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Endotoxins
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Hemolysin Proteins
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Recombinant Proteins
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insecticidal crystal protein, Bacillus Thuringiensis
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Green Fluorescent Proteins