Abstract
The cell line designated HHUS was established from human uterine cervical keratinizing squamous cell carcinoma. The HHUS cell line was subcultivated more than 70 times within 3 years. The cultured cells, polygonal or spindle, with neoplastic and pleomorphic features, appeared epithelial in shape, with a pavement-like arrangement and grew in multi-layers without contact inhibition. The chromosome number was varied from 40 to 88, and the modal number was stable in diploid range. The cultured cells produced keratinizing squamous cell carcinomas by heterotransplantation into the subcutis of nude mice. The HHUS cells were characterized as producing large amounts of SCC, in vitro and possessing HPV-59 DNA genomes.
MeSH terms
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Animals
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Antigens, Neoplasm / analysis
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / pathology*
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Carcinoma, Squamous Cell / virology*
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Cell Culture Techniques / methods*
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Cell Line, Tumor
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Chromosome Mapping
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Culture Media / chemistry
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Cytokines / analysis
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DNA, Viral / isolation & purification*
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Female
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Humans
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Mice
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Mice, Inbred BALB C
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Middle Aged
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Neoplasm Transplantation
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Papillomaviridae / classification
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Papillomaviridae / genetics
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Papillomaviridae / isolation & purification*
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Polymerase Chain Reaction
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Serotyping
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Uterine Cervical Neoplasms / genetics
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Uterine Cervical Neoplasms / pathology*
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Uterine Cervical Neoplasms / virology*
Substances
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Antigens, Neoplasm
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Culture Media
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Cytokines
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DNA, Viral