Abstract
Sca-1 (stem cell antigen-1) enriches for murine prostate cells capable of regenerating tubular structures containing basal and luminal cell lineages in a dissociated cell prostate regeneration system. Sca-1(+) fractions are enriched for cells at the G(0) stage of the cell cycle, and Sca-1(+) cells cluster in the proximal region of prostatic tubules where replication-quiescent cells have been localized. Castration-induced enrichment for androgen-independent cells results in a concomitant enrichment for Sca-1(+) cells. Genetic perturbations of PTEN/AKT signaling in prostate-regenerating cells leads to the initiation of tumorigenesis, and cancer progression is associated with a dramatic increase in Sca-1(+) cells. Sca-1-enriched prostate-regenerating cells possess multiple stem/progenitor cell properties and can serve as targets for cancer initiation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / metabolism
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Androgens / metabolism
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Animals
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Antigens, Neoplasm
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Biomarkers, Tumor / metabolism*
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Cell Separation
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Cell Transformation, Neoplastic
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Disease Models, Animal
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Disease Progression
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Flow Cytometry
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GPI-Linked Proteins
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Immunohistochemistry
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Magnetics
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Male
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Membrane Glycoproteins / metabolism*
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Mice
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Mice, Inbred C57BL
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Mice, SCID
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Microscopy, Confocal
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Microscopy, Fluorescence
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Multigene Family
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Neoplasm Proteins / metabolism*
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Neoplasms / pathology
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Prostate / metabolism*
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Prostatic Neoplasms / genetics*
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Prostatic Neoplasms / metabolism*
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Receptors, Androgen / metabolism
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Regeneration
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Signal Transduction
Substances
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Actins
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Androgens
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Antigens, Neoplasm
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Biomarkers, Tumor
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GPI-Linked Proteins
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Membrane Glycoproteins
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Neoplasm Proteins
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Psca protein, mouse
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Receptors, Androgen