Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method

Tom Geens; Ann Desplanques; Marnix Van Loock; Brigitte M Bönner; Erhard F Kaleta; Simone Magnino; Arthur A Andersen; Karin D E Everett; Daisy Vanrompay

doi:10.1128/JCM.43.5.2456-2461.2005

Sequencing of the Chlamydophila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method

J Clin Microbiol. 2005 May;43(5):2456-61. doi: 10.1128/JCM.43.5.2456-2461.2005.

Authors

Tom Geens¹, Ann Desplanques, Marnix Van Loock, Brigitte M Bönner, Erhard F Kaleta, Simone Magnino, Arthur A Andersen, Karin D E Everett, Daisy Vanrompay

Affiliation

¹ Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Coupure Links 653, 9000 Ghent, Belgium. [email protected]

Abstract

Twenty-one avian Chlamydophila psittaci isolates from different European countries were characterized using ompA restriction fragment length polymorphism, ompA sequencing, and major outer membrane protein serotyping. Results reveal the presence of a new genotype, E/B, in several European countries and stress the need for a discriminatory rapid genotyping method.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Outer Membrane Proteins / genetics*
Birds
Chlamydophila psittaci / classification
Chlamydophila psittaci / genetics*
Chlamydophila psittaci / isolation & purification
Europe
Genotype
Humans
Molecular Sequence Data
Phylogeny
Polymorphism, Restriction Fragment Length
Sensitivity and Specificity
Serotyping / methods

Substances

Bacterial Outer Membrane Proteins
OMPA outer membrane proteins

Associated data

GENBANK/AY762608
GENBANK/AY762609
GENBANK/AY762610
GENBANK/AY762611
GENBANK/AY762612
GENBANK/AY762613