Distribution of IS1311 and IS1245 in Mycobacterium avium subspecies revisited

Tone Bjordal Johansen; Berit Djønne; Merete R Jensen; Ingrid Olsen

doi:10.1128/JCM.43.5.2500-2502.2005

Distribution of IS1311 and IS1245 in Mycobacterium avium subspecies revisited

J Clin Microbiol. 2005 May;43(5):2500-2. doi: 10.1128/JCM.43.5.2500-2502.2005.

Authors

Tone Bjordal Johansen¹, Berit Djønne, Merete R Jensen, Ingrid Olsen

Affiliation

¹ Department of Animal Health, National Veterinary Institute, Pb 8156 Dep., 0033 Oslo, Norway.

Abstract

We demonstrated that IS1245 is not present in Mycobacterium avium subsp. paratuberculosis by restriction fragment length polymorphism and that the designated three-banded bird pattern of IS1245 in M. avium subsp. avium consists of one copy of IS1245 and two copies of IS1311. Cross hybridization between the two elements can be avoided by using more specific probes.

MeSH terms

Animals
Base Sequence
DNA Primers
DNA Transposable Elements / genetics*
Humans
Mycobacterium avium / classification*
Mycobacterium avium / genetics*
Mycobacterium avium / isolation & purification
Mycobacterium avium subsp. paratuberculosis / classification*
Mycobacterium avium subsp. paratuberculosis / genetics*
Mycobacterium avium subsp. paratuberculosis / isolation & purification
Polymorphism, Restriction Fragment Length
Species Specificity

Substances

DNA Primers
DNA Transposable Elements