Quality control in combinatorial chemistry: determinations of amounts and comparison of the "purity" of LC-MS-purified samples by NMR, LC-UV and CLND

J Comb Chem. 2005 May-Jun;7(3):364-71. doi: 10.1021/cc049850r.

Abstract

The absolute purities of 20 purified samples from a combinatorial library have been determined by a new method that uses the DMSO sidebands [1J[13C-1H]] as an internal standard for quantification. The obtained absolute amounts are compared with the amounts of sample obtained by weighing, with the calculated weights obtained by chemiluminescent nitrogen detection (CLND) chromatography and with the relative purities obtained by LC-UV chromatography.

Publication types

  • Comparative Study

MeSH terms

  • Chromatography
  • Chromatography, Liquid
  • Combinatorial Chemistry Techniques*
  • Dimethyl Sulfoxide / chemistry
  • Luminescent Measurements
  • Magnetic Resonance Spectroscopy
  • Nitrogen / analysis
  • Pharmaceutical Preparations / analysis*
  • Quality Control
  • Reference Standards
  • Reproducibility of Results
  • Spectrophotometry, Ultraviolet
  • Time Factors
  • Weights and Measures

Substances

  • Pharmaceutical Preparations
  • Nitrogen
  • Dimethyl Sulfoxide