Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus

Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1027-1031. doi: 10.1099/ijs.0.63489-0.

Abstract

A moderately halophilic, Gram-positive, rod-shaped bacterium (BH030004(T)) was isolated from a solar saltern in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BH030004(T) belonged to the genus Pontibacillus. Chemotaxonomic data (DNA G + C content, 42 mol%; major isoprenoid quinone, MK-7; cell-wall type, A1gamma-type meso-diaminopimelic acid; major fatty acids, iso-C(15 : 0) and anteiso-C(15 : 0)) also supported the affiliation of the isolate to the genus Pontibacillus. Although the 16S rRNA gene sequence similarity between strain BH030004(T) and Pontibacillus chungwhensis DSM 16287(T) was relatively high (99.1 %), physiological properties and DNA-DNA hybridization (about 7 % DNA-DNA relatedness) allowed genotypic and phenotypic differentiation of strain BH030004(T) from the type strain of P. chungwhensis. Therefore, strain BH030004(T) represents a novel species of the genus Pontibacillus, for which the name Pontibacillus marinus sp. nov. is proposed. The type strain is BH030004(T) (=KCTC 3917(T) = DSM 16465(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillaceae / classification*
  • Bacillaceae / cytology
  • Bacillaceae / isolation & purification*
  • Bacillaceae / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • Cyanoacrylates / analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diaminopimelic Acid / analysis
  • Diaminopimelic Acid / chemistry
  • Fatty Acids / analysis
  • Flagella
  • Genes, rRNA
  • Gentian Violet
  • Korea
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenazines
  • Phylogeny
  • Quinones / analysis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Sodium Chloride / pharmacology
  • Spores, Bacterial / cytology
  • Water Microbiology

Substances

  • Cyanoacrylates
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Gram's stain
  • Phenazines
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • cyacrin
  • Sodium Chloride
  • Diaminopimelic Acid
  • Gentian Violet

Associated data

  • GENBANK/AY603977