Abstract
Physiological characters, mating compatibility, PCR-RAPD fingerprints, mol% G + C content, DNA-DNA relatedness, and large-subunit and internal transcribed spacer rRNA gene sequences of strains assigned to the genus Zygoascus were re-examined. On the basis of those data, and after phylogenetic analyses, an emendation of Zygoascus hellenicus (type material is a cross of CBS 6736(T) x CBS 5839(T)) is proposed, comprising two novel anamorphic varieties, Candida steatolytica var. steatolytica (CBS 6736(T)) and C. steatolytica var. inositophila (CBS 5839(T)). A novel teleomorphic species, Zygoascus meyerae sp. nov. (type material is a cross of CBS 4099(T) x CBS 7521(T)) is described, together with two novel anamorphic varieties corresponding to it, Candida hellenica var. hellenica (CBS 4099(T)) and C. hellenica var. acidophila (CBS 7115(T)).
MeSH terms
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Base Composition
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Candida / classification
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DNA Fingerprinting
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DNA, Fungal / chemistry
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DNA, Fungal / isolation & purification
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / isolation & purification
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DNA, Ribosomal Spacer / genetics
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Genes, Fungal
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Genes, rRNA
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Molecular Sequence Data
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Mycological Typing Techniques
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Nucleic Acid Hybridization
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Phylogeny
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RNA, Fungal / genetics
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RNA, Ribosomal, 28S / genetics
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Random Amplified Polymorphic DNA Technique
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Saccharomycetales / classification*
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Saccharomycetales / cytology
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Saccharomycetales / genetics
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Saccharomycetales / physiology
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Sequence Analysis, DNA
Substances
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DNA, Fungal
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DNA, Ribosomal
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DNA, Ribosomal Spacer
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RNA, Fungal
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RNA, Ribosomal, 28S
Associated data
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GENBANK/AY447014
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GENBANK/AY447015
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GENBANK/AY447016
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GENBANK/AY447017
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GENBANK/AY447018
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GENBANK/AY447030
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GENBANK/AY447031
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GENBANK/AY447032
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GENBANK/AY447033
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GENBANK/AY447034