Abstract
Receptor-mediated gene transfer using an M cell ligand has been shown to be an efficient method for mucosal DNA immunization. To investigate further into alternative M cell ligands, the plant lectin, Ulex europaeus agglutinin I (UEA-1), was tested. UEA-1 binds to human intestinal Caco-2 cells, and these cells can be transfected with poly-l-lysine (PL)-conjugated UEA-1 for expression of reporter cDNAs. When tested in vivo, mice nasally immunized with UEA-1-PL complexed to plasmid encoding HIV-1 envelope showed elevated systemic and mucosal antibody responses, and these were supported by tissue antibody-forming cells. Likewise, elevated envelope-specific CTLs were induced. Thus, UEA-1 mediated DNA delivery represents an alternative mucosal formulation for inducing humoral and cellular immunity against HIV-1.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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AIDS Vaccines / immunology*
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Administration, Intranasal
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Animals
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Caco-2 Cells
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HIV Antibodies / analysis
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HIV Envelope Protein gp160 / genetics
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HIV Envelope Protein gp160 / immunology*
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HIV-1 / immunology*
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Humans
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Immunity, Mucosal*
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Immunoglobulin A / analysis
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Immunoglobulin G / blood
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Mice
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Mice, Inbred BALB C
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Mucous Membrane / immunology
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Plant Lectins / administration & dosage
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Plant Lectins / chemistry
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Plant Lectins / immunology*
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T-Lymphocytes, Cytotoxic / immunology
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Transfection
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Ulex*
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Vaccines, DNA / immunology*
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Vaccines, Synthetic
Substances
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AIDS Vaccines
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HIV Antibodies
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HIV Envelope Protein gp160
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Immunoglobulin A
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Immunoglobulin G
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Plant Lectins
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Ulex europaeus lectins
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Vaccines, DNA
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Vaccines, Synthetic