Abstract
To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algLdelta::Gm(r) mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.
MeSH terms
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Alginates / metabolism
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Genetic Complementation Test
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Glucuronic Acid / biosynthesis*
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Glucuronic Acid / metabolism
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Hexuronic Acids / metabolism
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Mutation
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Polymers / metabolism
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Polysaccharide-Lyases / genetics
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Polysaccharide-Lyases / metabolism*
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Pseudomonas aeruginosa / enzymology*
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Pseudomonas aeruginosa / genetics
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Uronic Acids / metabolism
Substances
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Alginates
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Hexuronic Acids
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Polymers
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Uronic Acids
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Glucuronic Acid
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Polysaccharide-Lyases
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poly(beta-D-mannuronate) lyase