Signaling pathways involved in tumor necrosis factor alpha-induced upregulation of the taurine transporter in Caco-2 cells

FEBS Lett. 2005 Jun 6;579(14):3069-74. doi: 10.1016/j.febslet.2005.04.063.

Abstract

We have previously demonstrated that the taurine uptake and transporter (TAUT) mRNA expression were upregulated by tumor necrosis factor alpha (TNF-alpha) in Caco-2 cells. In this present study, the signaling molecules related to this upregulation were investigated. Pyrrolidine dithiocarbamate, a nuclear factor kappaB (NF-kappaB) inhibitor, repressed the upregulation of taurine uptake and TAUT mRNA expression. A reporter assay revealed that TNF-alpha-induced TAUT transcriptional activity through the NF-kappaB consensus-like sequence in the human TAUT promoter region. An electrophoretic mobility shift assay showed that NF-kappaB could bind to the NF-kappaB consensus-like sequence. The anti-TNF receptor 1 (TNFR1) antibody, but not the TNF receptor 2 (TNFR2) antibody, repressed this upregulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Caco-2 Cells
  • Consensus Sequence / genetics
  • Humans
  • I-kappa B Proteins / metabolism
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism*
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism*
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism
  • Proline / analogs & derivatives*
  • Proline / pharmacology
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Tumor Necrosis Factor, Type I / metabolism
  • Receptors, Tumor Necrosis Factor, Type II / metabolism
  • Signal Transduction / drug effects*
  • Taurine / pharmacology
  • Thiocarbamates / pharmacology
  • Transcriptional Activation / drug effects
  • Transcriptional Activation / genetics
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Up-Regulation / drug effects*

Substances

  • I-kappa B Proteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • NF-kappa B
  • NFKBIA protein, human
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor, Type I
  • Receptors, Tumor Necrosis Factor, Type II
  • Thiocarbamates
  • Tumor Necrosis Factor-alpha
  • prolinedithiocarbamate
  • NF-KappaB Inhibitor alpha
  • taurine transporter
  • Taurine
  • Proline