Abstract
We have investigated the effect of bryostatin-1 (Bryo-1) and calcium ionophore (CI) on maturation and functions of DCs generated from adherent cells of cord blood cultured with GM-CSF plus IL-4 (CB-DCs). The CB-DCs treated with Bryo-1+CI exhibited morphologic characteristics of mature DCs, expressed increased levels of CD1a, CD80, CD83, CD86, and MHC class II as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN-gamma production. Treatment of CB-DCs with TNF-alpha or PMA+CI was less effective. Thus, Bryo-1+CI promotes maturation of CB-DCs and therefore could be used to enhance the neonatal immune response.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bryostatins
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Calcium / metabolism*
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Cell Differentiation / drug effects*
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Cells, Cultured
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Dendritic Cells / cytology
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Dendritic Cells / drug effects*
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Dendritic Cells / immunology
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Fetal Blood / cytology*
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Humans
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Infant, Newborn
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Interferon-gamma / biosynthesis
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Ionophores / pharmacology*
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Lymphocyte Activation / immunology
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Lymphocyte Culture Test, Mixed
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Macrolides / pharmacology*
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Monocytes / cytology
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Phenotype
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects*
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T-Lymphocytes / immunology
Substances
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Bryostatins
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Ionophores
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Macrolides
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bryostatin 1
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Interferon-gamma
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Calcium