SECISDesign: a server to design SECIS-elements within the coding sequence

Bioinformatics. 2005 Aug 1;21(15):3312-3. doi: 10.1093/bioinformatics/bti507. Epub 2005 May 26.

Abstract

Summary: SECISDesign is a server for the design of SECIS-elements and arbitrary RNA-elements within the coding sequence of an mRNA. The element has to satisfy both structure and sequence constraints. At the same time, a certain amino acid similarity to the original protein has to be kept. The designed sequence can be used for recombinant expression of selenoproteins in Escherichia coli.

Availability: The server is available at http://www.bio.inf.uni-jena.de/Software/SECISDesign/index.html.

Publication types

  • Evaluation Study

MeSH terms

  • Computer-Aided Design
  • DNA Transposable Elements / genetics
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Humans
  • Internet
  • Protein Engineering / methods*
  • Proteins / genetics*
  • Proteins / metabolism*
  • Recombinant Proteins / biosynthesis
  • Selenoproteins
  • Sequence Analysis, Protein / methods*
  • Sequence Analysis, RNA / methods*
  • Software*

Substances

  • DNA Transposable Elements
  • Proteins
  • Recombinant Proteins
  • Selenoproteins