Removing proteins from an aerated yeast fermentation by pulsing carbon dioxide: replacing salting-out as a method of protein precipitation

Appl Biochem Biotechnol. 2005 Spring:121-124:685-93. doi: 10.1385/abab:122:1-3:0685.

Abstract

Salting-out is a common technique used for precipitating proteins and other materials from fermentation and tissue culture processes. It leaves a salt residue in the system. Foam fractionation can also be used to remove proteins by protein precipitation from a dilute solution. In doing so, there is usually a trade-off between enrichment and recovery. An increase in the airflow rate will increase the recovery, but only at the expense of the enrichment. A new method for increasing the recovery in foam fractionations and in yeast fermentations is to add a burst of CO2 to the process and then restore the air. This CO2 acts like a temporary salt, but it does not leave behind a residue. The recovery increases as a result of the joint use of these gases, perhaps by more than 10-fold, without sacrificing the enrichment. Chicken egg albumin in a foam fractionation column can serve as a simple, experimental model for the proposed recovery process in lieu of the fermentation process.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Air*
  • Carbon Dioxide / chemistry*
  • Cell Culture Techniques / methods*
  • Fractional Precipitation*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / biosynthesis
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / isolation & purification*

Substances

  • Saccharomyces cerevisiae Proteins
  • Carbon Dioxide