Abstract
The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4(+) T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Antigens, CD / immunology
-
Bacterial Proteins / chemistry
-
CD4-Positive T-Lymphocytes / cytology
-
CD4-Positive T-Lymphocytes / immunology*
-
Cell Communication / immunology*
-
Cell Movement / immunology
-
Dendritic Cells / cytology
-
Dendritic Cells / immunology*
-
Immune Tolerance / immunology*
-
Lectins, C-Type / immunology
-
Luminescent Proteins / chemistry
-
Lymph Nodes / cytology
-
Lymph Nodes / immunology
-
Lymphocyte Activation / immunology
-
Mice
-
Mice, Inbred C57BL
-
Mice, Transgenic
-
Microscopy, Confocal
-
Minor Histocompatibility Antigens
-
Ovalbumin / immunology
-
Receptors, Cell Surface / immunology
-
Specific Pathogen-Free Organisms
Substances
-
Antigens, CD
-
Bacterial Proteins
-
DEC-205 receptor
-
Lectins, C-Type
-
Luminescent Proteins
-
Minor Histocompatibility Antigens
-
Receptors, Cell Surface
-
yellow fluorescent protein, Bacteria
-
Ovalbumin