Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness

SungWoong Han; Chikashi Nakamura; Ikuo Obataya; Noriyuki Nakamura; Jun Miyake

doi:10.1016/j.bbrc.2005.04.059

Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness

Biochem Biophys Res Commun. 2005 Jul 8;332(3):633-9. doi: 10.1016/j.bbrc.2005.04.059.

Authors

SungWoong Han¹, Chikashi Nakamura, Ikuo Obataya, Noriyuki Nakamura, Jun Miyake

Affiliation

¹ Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.

PMID: 15925564
DOI: 10.1016/j.bbrc.2005.04.059

Abstract

We have previously demonstrated a new cell manipulation technology by using an atomic force microscope (AFM) and ultrathin needles, named nanoneedles. The nanoneedle is an AFM tip etched by a focused ion beam (FIB) and is sharpened from 200 to 800 nm in diameter. In this study, we have evaluated the proper diameter of a needle required for insertion into human cells over a long period without causing cell death, and achieved highly efficient gene expression method for human cells using a nanoneedle and an AFM.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Cell Division
Cell Line
Cell Shape
Cell Survival
Gene Expression*
Gene Transfer Techniques / instrumentation*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Microscopy, Atomic Force / instrumentation
Microscopy, Confocal
Needles
Plasmids / administration & dosage
Plasmids / genetics
Recombinant Proteins / genetics
Recombinant Proteins / metabolism

Substances

Recombinant Proteins
Green Fluorescent Proteins