Abstract
Enantiomers of (+/-) 5-[2 (R,S)-{[2-(o-ethoxyphenoxy) ethyl] amino} propyl]-2-methoxy-benzenesulfonamide (tamsulosin, drug frequently used in the treatment of prostate diseases) were separated by capillary electrophoresis (CE). An acidic background electrolyte (BGE) with sulfated-beta-cyclodextrin (S-beta-CD) was used to create a chiral separation environment. Baseline separation of the isomers was achieved during 5 min using cathodic electro-osmotic flow (EOF) (countercurrent mode). The quantification limits were 5.3 x 10(-6) moll(-1) for R-isomer and 5.7 x 10(-6) moll(-1) for S-isomer. The R.S.D. values of peak area were 0.54% for R-isomer and 0.75% for S-isomer. The results achieved enable determination of 0.5% of optical impurity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetic Acid / chemistry
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Acetonitriles / analysis
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Adrenergic alpha-Antagonists / analysis
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Adrenergic alpha-Antagonists / chemistry*
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Calibration
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Chromatography, High Pressure Liquid
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Dose-Response Relationship, Drug
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Electrolytes
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Electrophoresis
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Electrophoresis, Capillary / instrumentation
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Electrophoresis, Capillary / methods*
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Furans / analysis
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Furans / chemistry
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Models, Chemical
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Osmosis
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Reproducibility of Results
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Stereoisomerism
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Sulfonamides / analysis
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Sulfonamides / chemistry*
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Sulfur / chemistry
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Tamsulosin
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Time Factors
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beta-Cyclodextrins / analysis
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beta-Cyclodextrins / chemistry*
Substances
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Acetonitriles
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Adrenergic alpha-Antagonists
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Electrolytes
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Furans
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Sulfonamides
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beta-Cyclodextrins
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tetrahydrofuran
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Sulfur
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Tamsulosin
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betadex
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Acetic Acid
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acetonitrile