A CFTR chloride channel activator prevents HrpN(ea)-induced cell death in Arabidopsis thaliana suspension cells

Plant Physiol Biochem. 2005 Jun;43(6):567-72. doi: 10.1016/j.plaphy.2005.03.003. Epub 2005 Apr 12.

Abstract

Erwinia amylovora is a necrogenic bacterium that causes fire blight of the Maloideae subfamily of Roseacae, such as apple and pear. It provokes necrosis in aerial parts of susceptible host plants and the typical hypersensitive reaction in non-host plants. The secreted harpin, HrpN ea, is able by itself to induce an active cell death in non-host plants. Ion flux modulations were shown to be involved early in such processes but very few data are available on the plasma membrane ion channel activities responsible for the pathogen-induced ion fluxes. We show here that HrpN ea induces cell death in non-host Arabidopsis thaliana suspension cells. We further show that two cystic fibrosis transmembrane conductance regulator modulators, glibenclamide and bromotetramisole, can regulate anion channel activities and HrpN ea-induced cell death.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / drug effects
  • Arabidopsis / physiology*
  • Bacterial Outer Membrane Proteins / metabolism*
  • Bacterial Outer Membrane Proteins / pharmacology
  • Cell Culture Techniques
  • Cell Death
  • Cystic Fibrosis Transmembrane Conductance Regulator / agonists*
  • Erwinia amylovora / metabolism*
  • Glyburide / pharmacology
  • Ion Channel Gating
  • Tetramisole / pharmacology

Substances

  • Bacterial Outer Membrane Proteins
  • harpin protein, Erwinia amylovora
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Tetramisole
  • Glyburide