[Association of metabolic and DNA repair enzyme gene polymorphisms and DNA damage in coke-oven workers]

Zhonghua Yu Fang Yi Xue Za Zhi. 2005 May;39(3):164-7.
[Article in Chinese]

Abstract

Objective: To investigate the association of polymorphisms of metabolic and DNA repair enzyme genes and DNA damage in peripheral blood lymphocytes in coke-oven workers.

Methods: One hundred and forty-four coke-oven workers and 50 controls were recruited in this study. Urinary 1-hydroxypyrene (1-OHP) levels were measured as the internal dose of polycyclic aromatic hydrocarbons exposure. DNA damage was detected by alkaline comet assay, and the value of 1.74 was used as the cut-off value to determine whether the individual's DNA damage was positive. The genotypes of CYP1A1, CYP2E1, GSTP1, NQO1, mEH and XRCC1 were determined by PCR-based methods. With adjustment for urinary 1-OHP, age, sex, multiple analysis of covariance was used to study the association between genotypes and the ln-transformed olive TM and multiple logistic regression was used to calculate the adjusted OR and the 95% CI for the risk of DNA damage.

Results: In 144 coke-oven workers, with adjustment for urinary 1-OHP, coking history and sex, the olive TM was significantly higher with XRCC1 280His allele than those with Arg allele (5.6 vs. 2.8, P < 0.01). The subjects with XRCC1 280His allele also have significantly higher risk for DNA damage than subjects with Arg allele (adjusted OR = 2.66, 95% CI = 1.00-7.14, P = 0.05) and the subjects with GSTP1 104Val allele have higher risk for DNA damage than subjects with Ile allele (adjusted OR = 1.90, 95% CI = 0.94-3.85, P = 0.07).

Conclusion: XRCC1 and GSTP1 polymorphisms might influence the susceptibility of DNA damage in occupational PAH-exposed coke-oven workers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • Coke / poisoning*
  • Comet Assay
  • Cytochrome P-450 CYP1A1 / genetics
  • Cytochrome P-450 CYP2E1 / genetics
  • DNA Damage*
  • DNA Ligase ATP
  • DNA Ligases / genetics
  • DNA Repair Enzymes / genetics*
  • Female
  • Genotype
  • Glutathione S-Transferase pi / genetics
  • Humans
  • Logistic Models
  • Male
  • Middle Aged
  • Multivariate Analysis
  • NAD(P)H Dehydrogenase (Quinone) / genetics
  • Occupational Exposure / adverse effects
  • Occupational Exposure / analysis
  • Polymorphism, Genetic*

Substances

  • Coke
  • Cytochrome P-450 CYP2E1
  • Cytochrome P-450 CYP1A1
  • NAD(P)H Dehydrogenase (Quinone)
  • NQO1 protein, human
  • Glutathione S-Transferase pi
  • DNA Ligases
  • DNA Repair Enzymes
  • DNA Ligase ATP