Abstract
Most studies of HIV-1 drug resistance have examined subtype B viruses; fewer data are available from developing countries, where non-B subtypes predominate. We determined the prevalence of mutations at protease and reverse transcriptase drug resistance positions in antiretroviral drug-naive individuals in southern India. The pol region of the genome was amplified from plasma HIV-1 RNA in 50 patients. All sequences clustered with HIV-1 subtype C. All patients had at least one protease and/or RT mutation at a known subtype B drug resistance position. Twenty percent of patients had mutations at major protease inhibitor resistance positions and 100% had mutations at minor protease inhibitor resistance positions. Six percent and 14% of patients had mutations at nucleoside reverse transcriptase inhibitor and/or nonnucleoside reverse transcriptase inhibitor resistance positions, respectively. Larger scale studies need to be undertaken to better define the genotypic variation of circulating Indian subtype C viruses and their potential impact on drug susceptibility and clinical outcome in treated individuals.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Amino Acid Substitution
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Base Sequence
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Drug Resistance, Viral / genetics
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Female
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HIV Infections / virology*
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HIV Protease / genetics
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HIV Reverse Transcriptase / genetics
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HIV-1 / drug effects
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HIV-1 / genetics*
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HIV-1 / isolation & purification
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Humans
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India
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Male
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Middle Aged
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Molecular Sequence Data
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Mutation
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Phylogeny
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Polymorphism, Genetic*
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RNA, Viral / blood
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RNA, Viral / genetics
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Sequence Analysis, DNA
Substances
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RNA, Viral
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HIV Reverse Transcriptase
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HIV Protease
Associated data
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GENBANK/AY787487
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GENBANK/AY787488
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GENBANK/AY787489
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GENBANK/AY787490
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