The rapid onset of elasticity during the assembly of the bacterial cell-division protein FtsZ

Biochem Biophys Res Commun. 2005 Jul 29;333(2):508-16. doi: 10.1016/j.bbrc.2005.05.152.

Abstract

FtsZ, a prokaryotic homolog of eukaryotic tubulin, is a major constituent of the bacterial Z-ring, which contracts the cell wall during cell division. Because the mechanical properties of FtsZ are unknown, its function in the maintenance and constriction of the Z-ring is not well understood. Here, quantitative rheometry shows that, at physiological concentrations, FtsZ filaments form, extremely rapidly, highly elastic networks within physiological time scales ( approximately minutes), much faster than other major dynamic cytoskeletal filaments, including microtubule, actin, and vimentin in eukaryotes. FtsZ networks display a relatively low viscosity and a high resilience against shear stresses, as well as an elasticity that depends weakly on concentration, G approximately C(0.57), a power-law dependence consistent with crosslinked flexible filaments. Calcium, whose intracellular concentration increases during bacterial division, further enhances the elasticity of FtsZ networks through filament bundling, an effect that occurs in the presence of GTP, not GDP. These studies suggest that FtsZ filaments have the toughness to provide strong mechanical support for the maintenance and circumferential constriction of the bacterial Z-ring.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / analysis*
  • Actins / chemistry*
  • Animals
  • Bacterial Proteins / analysis
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / ultrastructure*
  • Calcium / chemistry*
  • Cell Cycle Proteins / analysis
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / ultrastructure
  • Chickens
  • Cytoskeletal Proteins / analysis
  • Cytoskeletal Proteins / chemistry*
  • Cytoskeletal Proteins / ultrastructure*
  • Elasticity
  • Escherichia coli / chemistry*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Multiprotein Complexes / analysis
  • Multiprotein Complexes / chemistry
  • Stress, Mechanical

Substances

  • Actins
  • Bacterial Proteins
  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • FtsZ protein, Bacteria
  • Multiprotein Complexes
  • Calcium