Abstract
We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / analysis
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
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ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
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Cell Differentiation
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Down-Regulation
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Humans
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Intermediate Filament Proteins / genetics
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Intermediate Filament Proteins / metabolism
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Nestin
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Neurons / chemistry
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Neurons / metabolism*
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Phenotype
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Stem Cells / chemistry
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Stem Cells / metabolism*
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Intermediate Filament Proteins
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NES protein, human
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Nerve Tissue Proteins
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Nestin
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RNA, Messenger