Application of carbon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interaction

Biosens Bioelectron. 2005 Jul 15;21(1):201-5. doi: 10.1016/j.bios.2004.08.030.

Abstract

Carbon nanotube sensors detected anti-hemagglutinin binding to immobilized hemagglutinins. An ultra-sensitive detection method for antibodies or antigens in serum is required. Hemagglutinins were immobilized on the reverse side of a carbon nanotube, thereby producing a source and a drain. Electrode pads covered each edge of the nanotube. The I-V curves between the source and the drain were measured after incubation of anti-hemagglutinins with immobilized hemagglutinins in a buffered solution on the reverse side of the nanotube. The sensitivity of the CNT sensor was higher than that of an ELISA system. This method constitutes a new tool to analyze interaction among biomolecules on a substrate.

MeSH terms

  • Animals
  • Antibodies, Viral / analysis*
  • Antibodies, Viral / metabolism
  • Biosensing Techniques*
  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • Hemagglutinins / genetics
  • Hemagglutinins / immunology*
  • Hemagglutinins / metabolism
  • Humans
  • Influenza A Virus, H9N2 Subtype / genetics
  • Microelectrodes
  • Nanotubes, Carbon*
  • Nickel / metabolism

Substances

  • Antibodies, Viral
  • Hemagglutinins
  • Nanotubes, Carbon
  • Nickel