Abstract
Our recent research has indicated that intracerebroventricular (ICV) administration of pituitary adenylate cyclase-activating polypeptide (PACAP) suppresses food intake in the goldfish. We therefore examined feeding- and fasting-induced changes in the expression of mRNAs for PACAP and its receptors in the goldfish brain, and the effect of ICV administration of synthetic PACAP on locomotor activity in the goldfish. Semiquantitative analysis revealed that the expression of mRNAs for PACAP and the PAC1 receptor was significantly increased by excessive feeding (daily food supply at >or=5% of the body weight [BW]) for 7 days. ICV administration of PACAP at 20 pmol/g BW induced a significant decrease in locomotor activity during the 60-min post-treatment observation period. These results suggest that PACAP may have an anorexigenic action via hypomotility in goldfish.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anorexia / chemically induced
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Appetite / drug effects
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Appetite / physiology*
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Appetite Depressants / metabolism
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Appetite Depressants / pharmacology
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Brain / drug effects
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Brain / metabolism*
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Eating / drug effects
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Eating / physiology
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Feeding Behavior / drug effects
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Feeding Behavior / physiology*
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Female
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Goldfish / genetics
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Goldfish / metabolism*
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Injections, Intraventricular
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Male
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Motor Activity / drug effects
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Motor Activity / physiology
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Nerve Growth Factors / genetics
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Nerve Growth Factors / pharmacology
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Nerve Growth Factors / physiology*
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Neuropeptides / genetics
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Neuropeptides / pharmacology
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Neuropeptides / physiology*
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Neurotransmitter Agents / genetics
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Neurotransmitter Agents / pharmacology
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Neurotransmitter Agents / physiology*
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Pituitary Adenylate Cyclase-Activating Polypeptide
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RNA, Messenger / metabolism*
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Receptors, Cell Surface / agonists
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Substances
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Appetite Depressants
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Nerve Growth Factors
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Neuropeptides
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Neurotransmitter Agents
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Pituitary Adenylate Cyclase-Activating Polypeptide
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I