Vitamin a deficiency in patients with common variable immunodeficiency

J Clin Immunol. 2005 May;25(3):275-80. doi: 10.1007/s10875-005-4090-6.

Abstract

Vitamin A, a naturally occuring antioxidant micronutrient, has immunomodulating effect in patients with immunodeficiency, including an influence on cytokine production and lymphocyte growth and functions. Vitamin A deficiency is associated with a shift from type 2 cytokines to predominantly type 1 cytokines. The aims of this study were to determine Vitamin A status in Common variable immunodeficiency (CVID) patients and the relationship between Vitamin A status and cytokines production. Serum Vitamin A, neopterin, TNF-alpha, IL-2, IL-4, and IL-10 levels were determined in 19 CVID patients and 15 healthy children. Effects of 9-cis retinal, Vitamin A derivative, on cytokines (TNF-alpha, IL-2, IL-4 and IL-10) production in lymphocytes were tested in vitro condition using lymphocyte cultures obtained from CVID patients and healthy children.Serum Vitamin A level in CVDI patients was, 21.1+/- 1.5 microg/dL, significantly (p < 0.001) lower than the value, 35.7+/- 1.8 microg/dL, observed in healthy children. Serum neopterin level in the patients was, 9.8+/- 2.9 nmol/L, higher (p < 0.05) than the value, 3.9+/- 0.7 nmol/L, observed in control group. Common variable immunodeficiency patients, serum IL-4 level was significantly (p < 0.05) lower than the value observed for healthy children. Serum TNF-alpha, IL-2 and IL-10 levels were similar in the patients and healthy children. Vitamin A derivative, 9-cis retinal, increased TNF-alpha and IL-4 production in cultured mononuclear cells obtained from control and CVID patients. Vitamin A derivative, also, increased IL-2 and Il-4 production in cultured mononuclear cells obtained from CVID patients. These results show that CVID patients have low serum Vitamin A levels and high serum neopterin levels. A supplementation with Vitamin A may have role in downregulation of inflammatory responses in CVID patients.

MeSH terms

  • Adolescent
  • Adult
  • Case-Control Studies
  • Cells, Cultured
  • Child
  • Child, Preschool
  • Common Variable Immunodeficiency / complications*
  • Cytokines / biosynthesis
  • Cytokines / blood
  • Diterpenes
  • Down-Regulation / drug effects
  • Female
  • Humans
  • Interleukin-4 / analysis
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism
  • Lymphocytes / drug effects
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Male
  • Retinaldehyde / pharmacology
  • Tumor Necrosis Factor-alpha / analysis
  • Vitamin A / blood
  • Vitamin A / pharmacology
  • Vitamin A Deficiency / etiology*

Substances

  • Cytokines
  • Diterpenes
  • Tumor Necrosis Factor-alpha
  • Vitamin A
  • Interleukin-4
  • 9-cis-retinal
  • Retinaldehyde