Abstract
A recently reported high-throughput screening strategy has been applied to the rapid selection of new water-soluble antioxidants that display strong protective activities. Based on a competitive immunoassay, a triple-screening procedure was used to evaluate the ability of different compounds to protect thymidine under different oxidative stresses. The pro-oxidant effect of norbadione A in the presence of iron was observed, while some pulvinic acid derivatives proved strongly protective during gamma radiolysis, UV irradiation, and Fenton-like oxidation.
MeSH terms
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4-Butyrolactone / analogs & derivatives
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4-Butyrolactone / chemistry
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Antioxidants / chemistry*
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Antioxidants / pharmacology*
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Carboxylic Acids / chemistry
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Chromatography, High Pressure Liquid
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Evaluation Studies as Topic
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Flavonoids / chemistry*
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Flavonoids / physiology
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Immunoassay
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Lactones / chemistry
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Oxidants / chemistry*
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Oxidants / pharmacology
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Oxidative Stress
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Phenols / chemistry*
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Phenylacetates / chemistry
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Polyphenols
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Quercetin / chemistry
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Reactive Oxygen Species
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Thymidine / chemistry*
Substances
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Antioxidants
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Carboxylic Acids
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Flavonoids
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Lactones
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Oxidants
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Phenols
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Phenylacetates
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Polyphenols
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Reactive Oxygen Species
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norbadione A
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Quercetin
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4-Butyrolactone
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pulvinic acid
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Thymidine