A two-dimensional YAC pooling strategy for library screening via STS and Alu-PCR methods

Nucleic Acids Res. 1992 May 25;20(10):2559-63. doi: 10.1093/nar/20.10.2559.

Abstract

We have developed a simple two-dimensional YAC pooling strategy to facilitate YAC library screening via STS and Alu-PCR approaches. The method has been implemented using the human total genomic YAC library of Olson and coworkers, and its validity tested by isolation of many chromosomes 19- and 21-specific YACs. The Alu-PCR approach is notable in that it is hybridization-based, such that PCR primer pairs do not need to be repeatedly synthesized and tested for each screening step.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Carcinoembryonic Antigen / genetics
  • Chromosomes, Fungal
  • Genomic Library*
  • Molecular Sequence Data
  • Muscular Dystrophies / genetics
  • Oligodeoxyribonucleotides / genetics
  • Polymerase Chain Reaction*
  • Repetitive Sequences, Nucleic Acid / genetics*
  • Sequence Tagged Sites*

Substances

  • Carcinoembryonic Antigen
  • Oligodeoxyribonucleotides