The step-size defined as the movement between actin and myosin filaments per ATP split is an important issue in the research of muscle contraction. According to the conventional swinging-cross-bridge theory by Huxley, the step-size in this sense should be within 40 nm, a maximum possible stroke size of a myosin head, 20 nm, swing through an arc of 180 degrees . Since 1996, the estimated value over 40 nm has not been reported. Recently, however, there have been several discussions based on the one of the smallest values of step-size, 1.7 nm. We carefully re-evaluated the estimation process for the step-size, concluding that the values are still limited within 10-20 nm of conventional swinging-cross-bridge theory: 12.6 nm at no load for frog muscle.