[Induction of apoptosis in human hepatoma cell line SMMC7721 by Newcastle disease virus HN gene]

Zhonghua Zhong Liu Za Zhi. 2005 May;27(5):279-82.
[Article in Chinese]

Abstract

Objective: To investigate the mechanisms of apoptosis induced in human hepatoma cell line SMMC7721 by plasmid pVHN constructed with Newcastle disease virus (NDV) HN gene.

Methods: Twenty-four h after transfection with liposome-plasmid pVHN complexes in vitro, the mortality rate of SMMC7721 cells was determined by MTT staining and flow cytometry (FCM) with PI staining. The alteration of mitochondrial trans-membrane potential of the cells was detected by FCM with rhodamine 123 staining. Cell genomic DNA was detected by agarose electrophoresis. The activation of caspase-3 was assayed by its substrate color reaction.

Results: Significant apoptosis was induced by transfection with plasmid pVHN into the cells for 24 h and the mortality rate was 50.0% (the mortality rate of control group was 5.2%). Genomic DNA was fragmented and mitochondrial trans-membrane potential was decreased, but caspase-3 activity increased.

Conclusion: Significant apoptosis in SMMC7721 cells can be induced by NDV HN gene. Apoptosis may be resulted from the decrease of mitochondrial trans-membrane potential and activation of Caspase-3.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / physiology*
  • Cancer Vaccines / immunology
  • Carcinoma, Hepatocellular / pathology*
  • Caspase 3 / metabolism
  • Cell Line, Tumor
  • HN Protein / genetics*
  • Humans
  • Liver Neoplasms / pathology*
  • Newcastle disease virus / genetics*
  • Transfection
  • Vaccines, DNA / immunology

Substances

  • Cancer Vaccines
  • HN Protein
  • Vaccines, DNA
  • Caspase 3