Nosocomial viral ventilator-associated pneumonia in the intensive care unit: a prospective cohort study

Intensive Care Med. 2005 Aug;31(8):1116-22. doi: 10.1007/s00134-005-2706-1. Epub 2005 Jul 6.

Abstract

Objective: To determine the incidence, risk factors, and clinical relevance of viral ventilator-associated pneumonia (VAP) in an adult intensive care unit (ICU).

Design: Prospective observational study.

Setting: A 22-bed adult medical ICU in a university hospital.

Patients: All consecutive adult patients ventilated more than 48 h in a 9-month period including regular seasonal viral infections.

Interventions: A tracheobronchial aspirate upon enrollment and at the time of VAP suspicion.

Measurements and results: All respiratory specimens were tested in culture, indirect immunofluorescence assay, and PCR or RT-PCR for virological assessment. Patients were followed until ICU discharge or death. One hundred thirty-nine patients were included. Upon enrollment, a respiratory virus was detected in the tracheobronchial aspirate in 25% of patients (35 of 139). The incidence of VAP, defined according to clinical daily evaluation, was 28% (39 of 139 patients). A bacteria was documented in 74% of cases, whereas no case of a causative viral infection was encountered among VAP patients; however, herpes simplex virus type-1 (HSV 1) infection was detected in respiratory specimens of 31% of VAP (12 of 39).

Conclusions: We found a high incidence of HSV-1 infection in VAP patients; however, nosocomial viral VAP is likely to be rare in ICU, as assessed by the absence of respiratory virus-induced VAP identified in this prospective cohort study.

MeSH terms

  • Adult
  • Aged
  • Cohort Studies
  • Cross Infection / epidemiology
  • Cross Infection / etiology*
  • Cross Infection / virology
  • DNA Viruses / genetics
  • DNA Viruses / isolation & purification
  • Equipment Contamination
  • Female
  • France / epidemiology
  • Hospitals, University
  • Humans
  • Incidence
  • Intensive Care Units
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Pneumonia, Viral / epidemiology
  • Pneumonia, Viral / etiology*
  • Polymerase Chain Reaction
  • Prospective Studies
  • RNA Viruses / genetics
  • RNA Viruses / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Risk Factors
  • Time Factors
  • Ventilators, Mechanical / adverse effects*