Abstract
A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detection of H. pylori and comparable to agar dilution for the detection of resistance to clarithromycin.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Biopsy
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Clarithromycin / pharmacology*
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Culture Media
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Drug Resistance, Bacterial*
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Fluorescent Dyes
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Helicobacter Infections / microbiology
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Helicobacter pylori / drug effects
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Helicobacter pylori / genetics
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Helicobacter pylori / isolation & purification*
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Humans
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In Situ Hybridization, Fluorescence / methods*
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Microbial Sensitivity Tests
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Paraffin Embedding
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Reagent Kits, Diagnostic*
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Sensitivity and Specificity
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Stomach
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Time Factors
Substances
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Anti-Bacterial Agents
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Culture Media
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Fluorescent Dyes
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Reagent Kits, Diagnostic
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Clarithromycin