Detection of acid-fast bacilli in postlysis debris of clinical specimens improves the reliability of PCR

J Clin Microbiol. 2005 Jul;43(7):3580-1. doi: 10.1128/JCM.43.7.3580-3581.2005.
No abstract available

Publication types

  • Evaluation Study
  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques
  • Chelating Agents / chemistry
  • Chelating Agents / pharmacology
  • DNA, Bacterial / analysis
  • DNA, Bacterial / isolation & purification*
  • Guanidines
  • Humans
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification*
  • Octoxynol / pharmacology
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Specimen Handling / methods*
  • Sputum / microbiology*
  • Thiocyanates
  • Tuberculosis / diagnosis
  • Tuberculosis / microbiology

Substances

  • Chelating Agents
  • DNA, Bacterial
  • Guanidines
  • Thiocyanates
  • guanidine thiocyanate
  • Octoxynol