Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Sebastian R Sørensen; Jim Rasmussen; Carsten S Jacobsen; Ole S Jacobsen; René K Juhler; Jens Aamand

doi:10.1128/AEM.71.7.4144-4148.2005

Elucidating the key member of a linuron-mineralizing bacterial community by PCR and reverse transcription-PCR denaturing gradient gel electrophoresis 16S rRNA gene fingerprinting and cultivation

Appl Environ Microbiol. 2005 Jul;71(7):4144-8. doi: 10.1128/AEM.71.7.4144-4148.2005.

Authors

Sebastian R Sørensen¹, Jim Rasmussen, Carsten S Jacobsen, Ole S Jacobsen, René K Juhler, Jens Aamand

Affiliation

¹ Department of Geochemistry, Geological Survey of Denmark and Greenland, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. [email protected]

Abstract

A bacterial community from Danish agricultural soil was enriched with linuron [N-(3,4-dichlorophenyl)-N'-methoxy-N'-methylurea] as the sole carbon and nitrogen source. The community mineralized [ring-U-14C]linuron completely to 14CO2 and 14C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Comamonadaceae / classification*
Comamonadaceae / genetics*
Comamonadaceae / isolation & purification
Comamonadaceae / metabolism
Ecosystem
Electrophoresis / methods
Gram-Negative Bacteria / classification
Gram-Negative Bacteria / genetics
Gram-Negative Bacteria / isolation & purification
Gram-Negative Bacteria / metabolism*
Herbicides / metabolism*
Linuron / metabolism*
Molecular Sequence Data
Polymerase Chain Reaction / methods
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Soil Microbiology*

Substances

Herbicides
Linuron

Associated data

GENBANK/AY621157
GENBANK/AY621158