The study of the characteristic of photocytotoxicity under high peak power pulsed irradiation with ATX-S10Na(II) in vitro

Lasers Med Sci. 2005 Sep;20(2):54-61. doi: 10.1007/s10103-005-0342-1. Epub 2005 Jul 9.

Abstract

We studied hydrophilic photosensitizer ATX-S10Na(II) mediated photocytotoxicity against macrophage-like cell under pulsed irradiation. We found that photocytotoxicity suppression under high intensity irradiation was directly induced by a decrease in the Type-II photoreaction. We showed that this decrease was not attributable to absorption saturation with the high intensity irradiation. We found the cell lethality change from 70% to 13% with the pulse peak power density ranging from 0.29 MW/cm(2) to 1.36 MW/cm(2), at the light dose of 20 J/cm(2) and the pulse repetition rate at 40 Hz. To investigate the Type-II reaction, we measured the photobleaching, oxygen consumption and singlet oxygen luminescence of the photosensitizer solution. The transient absorption from the photosensitizer during the irradiation was measured with the pump-and-probe technique. We believe that the photocytotoxicity suppression induced by the high intensity irradiation might be useful for the treatment of depth-controlled photodynamic therapy without the wall damage of a hollow organ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Cell Survival / radiation effects
  • Dose-Response Relationship, Radiation
  • Luminescent Measurements
  • Macrophages / drug effects
  • Macrophages / radiation effects*
  • Mice
  • Oxygen Consumption / drug effects
  • Oxygen Consumption / radiation effects
  • Photochemotherapy*
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / pharmacology*
  • Porphyrins / chemistry
  • Porphyrins / pharmacology*
  • Singlet Oxygen / radiation effects
  • Spectrophotometry

Substances

  • ATX S10Na(II)
  • Photosensitizing Agents
  • Porphyrins
  • Singlet Oxygen