Objective: To explore the metabolic transformation and the absorbed metabolites of ophiopognin saponin D' (OD') given orally in rats.
Method: The contents of both original OD' and its metabolites were detected by means of HPLC-ELSD and the metabolites of OD' in blood and urine were measured by use of TLC and HPLC-MS in vivo.
Result: OD' could be metabolized by intestinal bacteria in rats. The content of diosgenin, one of the metabolites, increased gradually as the time passed.
Conclusion: OD' can be metabolized in intestine of rat and its metabolite, diosgenin, was absorbed in blood of rat.