The severe acute respiratory syndrome coronavirus 3a protein up-regulates expression of fibrinogen in lung epithelial cells

J Virol. 2005 Aug;79(15):10083-7. doi: 10.1128/JVI.79.15.10083-10087.2005.

Abstract

Here we analyzed the gene expression profile of cells that stably express the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein to determine its effects on host functions. A lung epithelial cell-line, A549, was chosen for this study because the lung is the primary organ infected by SARS-CoV and fatalities resulted mainly from pulmonary complications. Our results showed that the expression of 3a up-regulates the mRNA levels of all three subunits, Aalpha, Bbeta, and gamma, of fibrinogen. Consequently, the intracellular levels as well as the secretion of fibrinogen were increased. We also observed increased fibrinogen levels in SARS-CoV-infected Vero E6 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • Chlorocebus aethiops
  • Epithelial Cells / metabolism
  • Epithelial Cells / virology
  • Fibrinogen / genetics
  • Fibrinogen / metabolism*
  • Humans
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Severe acute respiratory syndrome-related coronavirus*
  • Up-Regulation
  • Vero Cells
  • Viral Envelope Proteins
  • Viral Proteins / metabolism*
  • Viroporin Proteins

Substances

  • 3a protein, SARS-CoV
  • RNA, Messenger
  • Viral Envelope Proteins
  • Viral Proteins
  • Viroporin Proteins
  • Fibrinogen