Abstract
Fibroblast Growth Factor-2 (FGF-2) induces cell proliferation, cell migration, embryonic development, cell differentiation, angiogenesis and malignant transformation. The four forms of FGF-2 (Low Molecular Weight) and (High Molecular Weights) are alternative translation products, and have a different subcellular localization: the high molecular weight (HMWFGF-2) forms are nuclear while the low molecular weight form, (LMWFGF-2) is mainly cytoplasmic. Our previous work demonstrated NIH 3T3 cells expressing different FGF-2 forms, displayed a different phenotype, suggesting that nuclear and cytoplasmic forms of FGF-2 may have different functions. Here we report a cDNA microarray-based study in NIH 3T3 fibroblasts expressing different FGF-2 forms. Several candidate genes that affect cell-cycle, tumor suppression, adhesion and transcription were identified as possible mediators of the HMWFGF-2 phenotype and signaling pattern. These results demonstrated that HMWFGF-2 and LMWFGF-2 target the expression of different genes. Particularly, our data suggest that HMWFGF-2 forms may function as inducers of growth inhibition and tumor suppression activities.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cluster Analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Early Growth Response Protein 1
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Fibroblast Growth Factor 2 / chemistry
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Fibroblast Growth Factor 2 / genetics*
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Fibroblast Growth Factor 2 / metabolism
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Gene Expression Profiling*
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Humans
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / metabolism
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Immunoblotting
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Mice
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Mitogen-Activated Protein Kinase 6 / genetics
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Mitogen-Activated Protein Kinase 6 / metabolism
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Molecular Weight
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NIH 3T3 Cells
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Oligonucleotide Array Sequence Analysis / methods
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Plasmids / genetics
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transfection
Substances
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DNA-Binding Proteins
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Early Growth Response Protein 1
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Egr1 protein, rat
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Immediate-Early Proteins
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Protein Isoforms
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RNA, Messenger
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Transcription Factors
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Fibroblast Growth Factor 2
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Mitogen-Activated Protein Kinase 6