Allele frequencies of 15 short tandem repeats (STRs) loci included in the PowerPlex 16 kit (Promega Corp., Madison, WI, USA) were obtained from a sample set of unrelated individuals inhabiting Rio Negro (N=593) and Chubut (N=320) provinces of the Argentinean Patagonia. Comparative analyses between our population data and other Patagonian Province (Neuquen) are presented.