Gene expression profiles in peripheral blood mononuclear cells from patients with subacute sclerosing panencephalitis using oligonucleotide microarrays

J Neurovirol. 2005 Jul;11(3):299-305. doi: 10.1080/13550280590953825.

Abstract

To investigate the molecular basis for measles virus persistence in patients with subacute sclerosing panencephalitis (SSPE), the authors used a high-density oligonucleotide microarray, and found that the expression of granulysin in peripheral blood mononuclear cells was significantly lower in the patients than in the controls. By a quantitative reverse transcriptase-polymerase chain reaction, the mRNA levels of granulysin were decreased in 30 SSPE patients, and were increased in 7 measles patients, as compared to the 23 controls. These results imply that granulysin might play a role in the host defense against measles virus and possibly be involved in the pathogenesis or pathophysiology of SSPE.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte / analysis
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Child
  • Female
  • Gene Expression Profiling*
  • Humans
  • Japan
  • Leukocytes, Mononuclear / metabolism*
  • Male
  • Oligonucleotide Array Sequence Analysis*
  • RNA, Messenger / genetics
  • Subacute Sclerosing Panencephalitis / metabolism*

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • GNLY protein, human
  • RNA, Messenger