Abstract
Objective:
To investigate the alteration of the gene HSD17B4 in esophageal squamous cell carcinoma and its potential significance.
Methods:
The mRNA expression and loss of heterozygosity (LOH) of HSD17B4 in 40 primary esophageal tumors were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and microsatellite analysis with the intragenic marker D5S1384 of the gene.
Results:
The frequencies of allelic loss of D5S1384 and the rate of down-regulation of gene HSD17B4 were 46.2% and 62.5%, respectively.
Conclusion:
HSD17B4 may be a candidate tumor suppressor gene associated with esophageal squamous cell carcinoma.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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17-Hydroxysteroid Dehydrogenases / biosynthesis
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17-Hydroxysteroid Dehydrogenases / genetics*
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Adult
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Aged
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Carcinoma, Squamous Cell / genetics*
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Down-Regulation
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Enoyl-CoA Hydratase / biosynthesis
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Enoyl-CoA Hydratase / genetics*
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Esophageal Neoplasms / genetics*
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Female
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Gene Expression
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Gene Expression Regulation, Neoplastic / genetics
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Genes, Tumor Suppressor
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Humans
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Hydro-Lyases
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Loss of Heterozygosity*
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Male
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Microsatellite Repeats
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Middle Aged
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Multienzyme Complexes / biosynthesis
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Multienzyme Complexes / genetics*
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Peroxisomal Multifunctional Protein-2
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Multienzyme Complexes
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RNA, Messenger
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17-Hydroxysteroid Dehydrogenases
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Hydro-Lyases
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Peroxisomal Multifunctional Protein-2
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HSD17B4 protein, human
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Enoyl-CoA Hydratase