Objective: To compare the PCR and isoenzyme methods in identification of Anopheles minimus A and C.
Methods: Samples of An. minimus collected in the field were identified from An. aconitus and An. jeyporiensis by PCR-RFLP after morphological examination, then classified by PCR-ASA. The mosquitoes identified by this method were further analyzed for isoenzyme to observe the difference, which was previously used as a technique to classify An. minimus A and C.
Results: PCR method can differentiate An. minimus A and C simply and quickly. For Isoenzyme method, only EST allozyme pattern displayed difference.
Conclusion: PCR method is better than isoenzyme analysis in differentiating An. minimus A and C.